Extraction and HPLC fingerprint analysis of the main substance of Ilex latifolia Thunb.
HU Ting1， ZOU Chengmei1， JIANG Jizhou1,2
1.School of Environmental Ecology and Biological Engineering， Wuhan Institute of Technology， Wuhan 430205， China；2. Key Laboratory of Rare Mineral， Ministry of Natural Resources， Geological Experimental Testing Center of Hubei Province， Wuhan 430034， China
Abstract：Through the preliminary optimization of the extraction method and conditions of the components of Ilex latifolia Thunb.， the optimum chromatographic separation conditions were selected by using high performance liquid chromatography (HPLC). The ultrasonic extraction method was selected from the types of principal components and the extraction rate of chemical components in the map， and 75% ethanol was determined as the extraction solvent with the ultrasonic time of 30 min. In the optimization of HPLC conditions， the mobile phase was selected as methanol-0.5% acetic acid according to the superiority of the final experimental results. The separation effect would be better achieved by gradient elution. 260 nm was used as the detection wavelength and the flow rate was 0.6 mL·min－1. The results of multiple methods showed that the extract solvent had good stability， and the common peak retention time of different batches was basically coincident after HPLC detection (RSD was 〈1% and similarity〉0.9)， which meets the requirements.
胡 婷,邹成梅,江吉周,. 苦丁茶主物质提取及其HPLC指纹图谱分析[J]. 华中师范大学学报(自然科学版), 2021, 55(1): 61-71.
HU Ting,ZOU Chengmei,JIANG Jizhou,. Extraction and HPLC fingerprint analysis of the main substance of Ilex latifolia Thunb.. journal1, 2021, 55(1): 61-71.
2021, Vol. 55 
