Study on determination of vitamin B12 in functional beverage by microbiological method
YAN Tao1,2， LIU Ping1,2， LI Juan1,2， CHEN Qiong1,2， CHEN Zhe1,2， ZHENG Nan3， ZENG Huijun1,2
(1.Wuhan Institute for Food and Cosmetic Control， Wuhan， 430040， China；2.Key Laboratory of Edible Oil Quality and Safety for State Market Regulation， Wuhan 430072， China； 3.Wuhan Polytechnic， Wuhan 430072， China)
Abstract：The determination of vitamin B12 is one of the most challenging problems in food analysis worldwide. A new method for detecting vitamin B12 in functional beverages was established. With reference to the determination method of vitamin B12 in infant foods and dairy products in the national food safety standard GB 5413.14－2010， which stems from the linear relationship between the growth rate of Lactobacillus leishmanii and B12 concentration， the standard curve was constructed and the content of vitamin B12 in samples were measured. Due to the differences among the substrates of functional beverages and milk powder， sample treatment methods were optimized based on the national standard. In addition， the quality control by standard additional recovery was conducted and the test result was compared with commercial assay kit. These results indicated that this method is able to be applied to the determination of vitamin B12 in functional beverages because of its simplicity， sensitivity and accuracy.
2022, Vol. 56 
