Abstract：Coomassie brilliant bule-G250 is able to react with bovine serum protein and form a blue complex. There was a corresponding relationship between protein concentration and product color depth. On the basis of error transfer law， the phone colorimetric method was used to investigate the external factors influencing sampling such as illumination and phone types. And a new method of protein content determination was established. Results showed that under the optimum conditions the standard deviation XB had a sinificant linear relationship with the concentration of protein at the linear range of 1～7 μg/mL. The relative standard deviation of standard sample determination was 3.47% (n＝8)， and the limit of detection was 0.67 μg/mL. The standard recovery experiment was conducted on the water-soluble protein of nuts such as peanuts， sunflower seed， pumpkin seed. The recovery was 100.3%～107.5%. This method had the advantages of simplicity， rapidity， high sensitivity and good recovery. and which would be used to determine the content of water-soluble protein in nuts.
周 佳,毕秀成,吴承明. 基于误差传递规律的手机比色法测定坚果中水溶性蛋白质[J]. 华中师范大学学报(自然科学版), 2019, 53(1): 57-62.
ZHOU Jia,BI Xiucheng,WU Chengming. Determination of water-soluble protein in nuts by mobile phone colorimetric based on error transfer law. journal1, 2019, 53(1): 57-62.
2019, Vol. 53 
