Abstract：In this study， precipitation fractionation dissolution method was used to purify the total triterpenes in Bergenia emeiensis and the antioxidant， anticancer as well as antibacterial activities were analyzed before and after purification. The results showed that after repeated freeze-dry， the content of triterpenes increased from 48.58% to 70.99% while the sample extraction rate lowered from 38.13% to 26.79%. The total reducing ability of the purified total triterpenes was promoted and the ability to scavenge DPPH free radicals was boosted with the IC50 changing from 0.32 mg·mL－1 to 0.27 mg·mL－1. The total triterpenes in Bergenia emeiensis could well inhibit Pseudomonas aeruginosa and Bacillus subtilis. After purification， inhibition zones of the total triterpenes on two strains enhanced. The IC50 to Hela cells was changed from 102.5 and 69.00 μg·mL－1 to 59.01 and 28.56 μg·mL－1 for 24 and 48 hours， respectively after purification. Moreover， the IC50 to A549 cells was changed from 238.90 and 172.00 μg·mL－1 to 84.32 and 65.99 μg·mL－1 for 24 and 48 hours， respectively. Therefore， the precipitation fractionation dissolution method can be applied to the purification of total triterpenes in Bergenia emeiensis and the purified total triterpenes exhibited a better antioxidant， anticancer and bacteriostatic activity in vitro.
罗思源,曾 辰,冯士令,陈 涛,周莉君,丁春邦. 峨眉岩白菜总三萜纯化及活性分析[J]. 华中师范大学学报(自然科学版), 2020, 54(5): 826-832.
LUO Siyuan,ZENG Chen,FENG Shiling,CHEN Tao,ZHOU Lijun,DING Chunbang. Purification of total triterpenes from Bergenia emeiensis and comparison of the bioactivities before and after purification. journal1, 2020, 54(5): 826-832.
2020, Vol. 54 
