Comparative analysis of aroma volatiles in different flowering stages and organs of Camellia handelii and Camellia uraku (Mak.) Kitamura
YANG Min1,2， LI Qingyuan1， XIANG Conglin1,4， CHEN Baolin1， FU Shao1， DU Kebin2， ZHANG Donglin3， XU Lin1
(1.Institute of Forestry and Pomology， Wuhan Academy of Agricultural Sciences， Wuhan 430075， China；2. College of Horticulture and Forestry， Huazhong Agricultural University， Wuhan 430070， China；3. Department of Horticulture， University of Georgia， Athens 30602， USA；4. Wuhan Jiangxia District Garden and Forestry Bureau， Wuhan 430200， China)
Abstract：Floral scent is an important horticultural trait of ornamental plants， while there is limited research on the scent of camellia at present. In this study， Camellia handelii and Camellia uraku (Mak.) Kitamura were used as the test materials. The release regularity and dynamic changes of volatiles in different parts of the organs (stamens， petals) were investigated. Based on headspace solid phase microextraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC-MS) technology， the volatile components of camellia flowers were analyzed. Internal standard method and retention index method were used in combination of Xcalibur software to match the peak map to the NIST standard spectral library， and the volatile components in the floral scent of camellia were explored. 50 and 29 volatile compounds were detected in the flowers of Camellia handelii and Camellia uraku (Mak.) Kitamura， respectively. The release amount showed a trend of first rising and then decreasing， all of which reached the peak during the blooming period， and the stamens were the main organs that released aroma. Among them， there are 23 kinds of volatile components of the petals of the flowering period of Camellia handelii(16.06 μg · g－1)， and 35 kinds of volatile components of stamens (42.78 μg · g－1). There are 13 kinds of volatile components of the petals of Camellia uraku (Mak.) Kitamura in full bloom (2.73 μg · g－1)， and 20 kinds of volatile components of stamens (16.00 μg · g－1). The two tested types of camellia flowers were found to contain 7 types of volatile compounds， including benzene rings/phenylpropanoids， aldehydes， terpenes， alcohols， ketones， hydrocarbons and esters. Among them， the total volatile components of Camellia handelii are mainly terpenoids， with phenethyl alcohol and α-ocimene being the most important volatile components， and sensory evaluation revealed a mixed fragrance of grassy， floral and sweet aromas. The total volatile components of Camellia uraku (Mak.) Kitamura are mainly terpenes， alcohols and ester compounds， with cis-3-nonene-1-alcohol and trans-linalool oxides (furans) being the most important volatile components. Despite having a floral fragrance by sensory evaluation， it exhibited low release and almost no aroma.
2023, Vol. 57 
