Optimum Mo concentration for promoting the symbiotic relationship between rhizobia strains and Stylosanthes guianensis Reyan No.5
DONG Rongshu, ZHANG Jie, CHEN Zhijian, BAI Changjun, HUAN Hengfu, WANG Wenqiang, LIU Guodao
Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan 571737
Abstract:The sand culture method was used to determine the optimum Mo concentration for promoting the symbiotic relationship between 4 rhizobia strains and S. guianensis cv. Reyan No.5 under 7 levels of Mo concentration. The relative plant height, overground yield, leaf nitrogen content, root nodule counts and nitrogenase activity were used as an integrated analysis to determine the optimum Mo concentration. Results showed that high Mo concentration was not effective to improve overground yield, Mo deficiency was not effective to improve leaf nitrogen content. Comprehensive analysis of the main components indicates that strain LZ3-2 at 1 500 mg·L-1, strains RJS9-2 at 1 000~1 500 mg·L-1, strains YM11-1 at 500~1 500 mg·L-1 and strains PN13-3 at 2 000 mg·L-1 could all effectively promotes nitrogen fixation for S. guianensis cv. Reyan No.5.