Abstract：The development of LNP delivery system provides new ideas for EV71 oncolytic virus. In order to enhance the delivery efficiency of EV71 oncolytic virus， EV71 liposome nanoparticles (EV71@ONP) were prepared by microfluidic encapsulation method and their particle size and encapsulation rate were detected by nanoparticle size potentiometer. By transfecting RD cells with EV71@ONP， the EV71 virus was successfully revived and produced. ELISA results didn’t show significant change in the TNF-a level in the supernatant of mouse monocyte macrophage RAW264.7 transfected with EV71@ONP， indicating that it has good activity and low immunogenicity. The cytotoxicity assay by CCK-8 showed that the survival rate of human colon cancer HT29 cells in the EV71@ONP (0.2 mg) treated group was significantly lower than that in the EV71 virus (MOI＝0.1) treated group at 24 h. Early and late apoptosis were detected in HT29 cells was anlayzed by flow cytometry while less toxic to human normal colon epithelial NCM460 cells was observed， indicating that EV71@ONP has a better growth inhibitory effect and safety of colon cancer cells in vitro. A tumor model of HT29-BALB/c nude mice was constructed， and it was found that EV71@ONP has a better growth inhibitory effect on the xenografts of experimental mice， suggesting its inhibitory effect in vivo. The above results indicated that this EV71 liposome nanoparticle encapsulation technology is expected to further improve the oncolytic effect of EV71 virus on human colon cancer and broaden the application of EV71 virus in cancer therapy.
孙维宝,郑小迪,张绥欣,张二帅,倪 鹏,汪 众,熊 丽. 脂质纳米颗粒封装EV71病毒的技术路线及其溶瘤效应初探[J]. 华中师范大学学报(自然科学版), 2025, 59(4): 577-584.
SUN Weibao,ZHENG Xiaodi,ZHANG Suixin,ZHANG Ershuai,NI Peng,WANG Zhong,XIONG Li. Preliminary study on the technical route of lipid nanoparticles to encapsulate EV71 virus genome and its oncolytic effect. journal1, 2025, 59(4): 577-584.
2025, Vol. 59 
