Physicochemical properties and hemostatic efficacy of oxidized regenerated cellulose （ORC） hemostatic aggregate： a comparative study
LI Huari1,2, LIU Dan1, MI Jun1, LI Na1
1.Department of Biochemistry and Molecular Cell Biology， Basic Medical Institute， Shanghai Jiao Tong University;School of Medicine， Shanghai 200025， China；2.Department of Biochemistry and Molecular Biology，College of Laboratory Medicine， Bengbu Medical University， Bengbu 233030， Anhui， China.
Abstract：This study evaluated an innovative hemostat-oxidized regenerated cellulose （ORC） aggregate derived from pine pulp-against two polysaccharide-based powders. Scanning electron microscopy revealed that the ORC aggregate has a particle size of 200-400 μm， significantly larger than the polysaccharide-based powders （10-45 μm and 40-90 μm）. In penetration tests， the ORC aggregate reached the bottom of the blood column within 1 min， while the polysaccharide-based powders remained floating on the surface for over an hour. All tested hemostats demonstrated hemolysis rates below the 5% safety threshold. In vitro coagulation assays showed that the ORC aggregate achieved whole blood coagulation index of 92.75% at 1.5 minutes and 98.06% at 3 min. In contrast， the polysaccharide-based powders failed to reach 10% even after 12 min. In an in vivo rabbit liver-bleeding model， the ORC aggregate achieved hemostasis within 3 min， significantly faster than the polysaccharide-based powders， which required more than 5 minutes. These results indicate that the ORC aggregate offers higher penetration， faster coagulation kinetics， and superior in vivo hemostatic efficacy， demonstrating its potential as a more effective and reliable hemostatic agent for surgical applications.
2025, Vol. 59 
