Abstract:The interaction of ultraviolet absorbent 2-phenyl benzimidazol-5-sulfonic acid (PBSA) and DNA in physiological condition was investigated by fluorescence spectrometry and UV absorption spectrophotometry. The results showed that PBSA interacted with DNA in an electrostatic interaction mode, which resulted in the hypochromic effect as well as fluorescence quenching of PBSA. The combination reaction of them was a single static quenching process, and the binding site was 1.11.
陈砚美,杨 晗,王 芳,熊绪杰,张万举. 紫外线吸收剂PBSA与DNA相互作用的光谱研究[J]. 华中师范大学学报(自然科学版), 2017, 51(3): 324-327.
CHEN Yanmei,YANG Han,WANG Fang,XIONG Xujie,ZHANG Wanju. Study on the interaction of ultraviolet absorbent PBSA with DNA by spectroscopic method. journal1, 2017, 51(3): 324-327.