Effect of total phenolics acid of Phellinus lonicerinus (Bond.)Bond. et Sing on hepatic fibrosis by regulating mast cells
ZHANG Hongqi1,2， LIU Wei1， WANG Junzhi2， LIU Zhiwen1， ZHANG Tao1， LUO Fei1
1.Medical College of China Three Gorges University， Yichang， Hubei 443002， China；2.Hubei Key Laboratory of Natural Products Research and Development， China Three Gorges University， Yichang， Hubei 443002， China
Abstract：To investigate the protective effect of total phenolics acid of Phellinus lonicerinus (Bond.) Bond. et Sing (PLTP) in resisting hepatic fibrosis by regulating mast cells. 50 rats were randomly divided into normal group， model group， colchicine group (0.2 mg/kg) ， PLTP-high and low dose group(500， 250 mg/kg). Rat hepatic fibrosis was induced by intraperitoneal injection of 50% CCl4-olive oil solution at a dose of 2 mL/kg twice weekly for 8 w. After the first injection， the normal group and model group were given equivoluminal physiological saline， and other groups were given the corresponding does of drugs once per day for 8 w. After the last administration， the rats were fasting for 24 h， and all the rats were killed with blood samples and liver tissues collected The serum contents of alanine aminotransferase (ALT)， aspartate aminotransferase (AST)， alkaline phosphatase (ALP)， hyaluronic acid (HA)， precollagen type Ⅲ (PCⅢ) and typeⅣ collagen (Ⅳ-C) were measured biochemical method. The α-smooth muscle actin (α-SMA) were detected by immunohistochemical assays. Meanwhile， the histopathological changes and mast cell (MC) of the liver tissue were observed. The results showed that the PLTP could significantly improve the liver tissue lesion， decrease the levels of ALT， AST， ALP， HA， PCⅢ and Ⅳ-C. The microexamination results of histological sections showed that PLTP significantly reduced the rat liver injury and fibrosis induced by CCl4. The CCl4 model group exhibited flatty degeneration and fibrosis with many degranulating and degranulated mast cells filled with purple granula located around the liver blood vessels and in fiber-interval， while those of PLTP-treated group decreased significantly. Immunohistochemical staining suggested that the expression of α-SMA was down-regulated with PLTP treatment. In summary， the PLTP possessed preventive and therapeutic effects on hepatic fibrosis induced by CCl4 in rats， and the mechanism might be related to the decrease of the number of mast cell and the expression of α-SMA in liver tissues.
2019, Vol. 53 
