Research of tissue culture in Gynura divaricate (L.) DC
LWIN Moe Moe1,2， ZHAO Zeyi3， HAN Boli3， YANG Wannian1, ZHANG Guobin4
1.School of Life Sciences， Central China Normal University， Wuhan 430079；2.Department of Botany， Yemanchaung Degree College， Yemanchaung, Myanmar；3.Wuhan Foreign Languages School， Wuhan 430200； 4.School of Life Sciences， WuChang University of Technology， Wuhan 430223
Abstract：The tender leaves of Gynura divaricata(L.)DC were used as the explants to research the regeneration system optimization of Eucommia. The results showed that callus inducement and callus proliferation grew well under the condition of MS＋1.0 mg/L NAA＋1.0 mg/L 6-BA＋0.75% Agar＋3% Sugar， with the induction rate of 100%. The growth cycle was 30 d at the process of callus proliferating. Growth curve appeared the shape of "S". The most suitable medium of buds inducement was MS＋1.0 mg/L NAA＋1.5 mg/L 6-BA. The induction rate was 100%. In a high concentration of 6-BA， the indefinite bud growth is weak. In the process of induced multiple shoots， adding suitable amount of IBA and GA is advantageous to the growth of multiple shoots， high concentration of NAA is conducive to root differentiation.
2017, Vol. 51 
