Isolation and identification of pathogenic fungi of pepper root rot and biological agents of Gauze No.15 in Shiyan
CAI Gaolei1,3， ZHAO Changsong1， PENG Xuanhe1， WU Pinghua1， ZHANG Fan1， WANG Hua2,3
1.Danjiangkou Reservoir of Shiyan Ecological Agriculture Institute， Shiyan， Hubei 442000， China；2.Institute of Plant Protection and Soil & Fertilizer， Hubei Academy of Agricultural Sciences， Wuhan 430064， China；3.Key Laboratory of Integrated Pest Management Crops in Central China， Ministry of Agriculture， Hubei Key Laboratory of Crop Disease， Insect Pests and Weeds Control， Wuhan 430064， China
Abstract：To identify the pathogen of pepper root rot， the pathogenic fungi was isolated and purified from pepper stalk root disease. The pathogenic fungus was verified via molecular determination and Koch's Rule. Field control and growth promotion effect were achieved to prevent pepper root rot by Gauze No.15. The result showed that the pathogen causing root rot of pepper was identified as Fusarium solani through identification of morphological characteristics and molecular biological analysis including ITS， EF-1a and IGS genes and phylogenetic tree in this area.Koch's Rule was finished by infestation test. In addition， the result indicated that applying 240 g/667 m2 of Paenibacillus polymyxa， 50 g/den and 75 g/den of Gause No.15 and 3% hymexazol metalaxyl AS (1 500×) generated the optimal inhibition effect on the Fusarium solani(more than 70%).After treatment， the pepper height， root length， fresh weight of the whole plant and root， and dry weight of the root had significant difference comparing with control group.
2019, Vol. 53 
