Sophoraflavanone G promote the expression and translocation of GLUT4 in L6 cells
SONG Guanjun1， TIAN Di1， ZHAO Ping1， YANG Xingzhou2
1.College of Life Sciences， South-Central University for Nationalities， Wuhan 430074， China；2.College of Pharmacy， South-Central University for Nationalities， Wuhan 430074， China
Abstract：In this study，the effects of Sophoraflavanone G (SFG) on glucose uptake were detected in L6 cells by cell glucose uptake assay kit. We found that SFG increased glucose uptake in L6 cells. Western blotting was used to detect the effects of SFG on GLUT4 expression level， and it was found that SFG significantly promoted GLUT4 expression in L6 cells. Meanwhile， the translocation of GLUT4 also was monitored. We found that SFG significantly promoted GLUT4 translocation， and the effects of SFG on the translocation of GLUT4 was concentration dependent in L6 cells. In addition， immunofluorescence test results also showed that SFG enhanced the fusion of GLUT4 and cell membrane in L6 cells.These results showed that SFG promoted glucose uptake by improving GLUT4 expression， translocation and fusion with cell membrane in L6 cell and SFG might have the potential to develop a new hypoglycemic drug.
宋冠军,田迪,赵平,杨新洲. 槐属二氢黄酮G促进L6细胞内GLUT4的表达和转运[J]. 华中师范大学学报(自然科学版), 2019, 53(3): 386-391.
SONG Guanjun,TIAN Di,ZHAO Ping,YANG Xingzhou. Sophoraflavanone G promote the expression and translocation of GLUT4 in L6 cells. journal1, 2019, 53(3): 386-391.
2019, Vol. 53 
