Abstract：Mammalian genomes are pervasively transcribed to produce thousands of long non-coding RNAs (lncRNA)， and lncRNA can regulate the gene expression through different manners， while only a few lncRNAs have been studied well and whose potential function and mechanism in mouse embryonic stem cells (mESCs) remain largely unknown. lnc1343 is an lncRNA transcribedby small nucleolar RNA host gene 3， and has been found dysregulated in many human diseases. Here， lnc1343 was reported to regulate the pluripotency of mESCs by the lncRNA transcripts themselves. CRISPR-cas9-mediated transcription start site and DNA locus knockout were utilized to construct the lnc1343-knockout cell lines in which the pluripotency genes Nanog， Sox2 and Oct4 decreased significantly. lnc1343expression also acted as local regulators which was correlated with the expression of nearby gene Rcc1. By analysis the Chip-seq data，it's revealed that several enhancer-like sequences dispersed throughout the lnc1343 and nearby gene. Moreover， this region was strongly enriched in enhancer markers， including H3K27ac and H3K4mel. Capture C assay was introduced to capture the interaction regions of lnc1343 and most of them located in the promoters of many different genes， which indicated the genomic locus of lnc1343 might be an enhancer-like region and regulate the gene expression by the long-range chromatin interaction. In summary， lnc1343 regulates the pluripotency by the lncRNA transcripts themselves， and the long-range chromatin interaction by its genomic locus.
陈绍恢,苏光松,陈 军,吕万革. lnc1343对小鼠胚胎干细胞多能性的影响及其基因座潜在作用机制[J]. 华中师范大学学报(自然科学版), 2021, 55(2): 258-269.
CHEN Shaohui,SU Guangsong,CHEN Jun,LYU Wange. Effect of lnc1343 on pluripotency of mouse embryonic stem cell and the potential mechanism of its DNA locus. journal1, 2021, 55(2): 258-269.
2021, Vol. 55 
