Abstract：In order to solve the problem of low catalyst activity in the preparation of (S)-omeprazole， a microemulsion system was used to prepare (S)-omeprazole from (S)-omeprazole thioether catalyzed by soybean pod peroxidase (SPP) in hexadecyl trimethyl ammonium bromide (CTAB)/chloroform/n-butanol/water-in-oil microemulsion. The response surface methodology (RSM) was used to optimize the reaction conditions， and the response surface model was established to obtain the optimal reaction conditions. The conversion rate， yield and enantiomeric excess percentage of (S)-omeprazole reached 93.75%， 91.56% and 96.08%， respectively. The experiment also demonstrated that SPP has low cost， good enantioselectivity， and good thermal stability， and it's able to replace the higher cost horseradish peroxidase (HRP) for biocatalytic reactions.
王志勇,李德鹏,李惠玲,王繁业. 微乳液中豆荚过氧化物酶催化制备(S)-奥美拉唑[J]. 华中师范大学学报(自然科学版), 2021, 55(4): 597-602.
WANG Zhiyong,LI Depeng,LI Huiling,WANG Fanye. Preparation of (S)-omeprazole catalyzed by soybean pod peroxidase in microemulsions. journal1, 2021, 55(4): 597-602.
2021, Vol. 55 
