Determination of hexanal and 2-butanone in human serum using magnetic in-tube solid phase micro-extraction coupled with high performance liquid chromatography
WANG Li， YAN Ling， HUANG Xiaolan， ZHOU Hongbin
(Key Laboratory of Pesticide and Chemical Biology， Ministry of Education， College of Chemistry， Central China Normal University， Wuhan 430079， China)
Abstract：In this study， C8 functionalized magnetic mesoporous silica spheres (C8-Fe3O4@mSiO2) was prepared and placed in a polytetrafloroethylene tube for magnetic in-tube micro-solid-phase extraction coupled with in-situ derivatization (MIT-MSPE-ISD) for the determination of hexanal and 2-butanone in human serum. Using a magnet to control the adsorbents moving along the inner wall of PTFE tube， the simultaneous extraction and derivation without any other pretreatment was acquired. Under optimized conditions， the limits of detection (LODs) were 1.0 and 1.2 μmol·L-1 for hexanal and 2-butanone， respectively. The relative recoveries ranged from 78.4% to 92.4% with the intra-day and inter-day relative standard deviations (RSDs) less than 7.9%. The proposed method was successfully applied to determine endogenous hexanal and 2-buanone in human serum from healthy persons and liver cancer patients.
王 莉,严 玲,黄小兰,周红斌. 磁性管内固相微萃取—液相色谱测定血清中己醛和2-丁酮[J]. 华中师范大学学报(自然科学版), 2022, 56(3): 460-466.
WANG Li,YAN Ling,HUANG Xiaolan,ZHOU Hongbin. Determination of hexanal and 2-butanone in human serum using magnetic in-tube solid phase micro-extraction coupled with high performance liquid chromatography. journal1, 2022, 56(3): 460-466.
2022, Vol. 56 
