Abstract：The polysaccharide TCP-C1-1 was extracted and isolated from the rhizome of Tupistra chinensis Baker， with its structure determined and biological activity studied. The polysaccharides extracted from rhizome of Tupistra chinensis Baker were extracted by water extraction and alcohol precipitation. The 80% alcohol precipitation part TCP-C was purified by Sevage method. The polysaccharide TCP-C1-1 was purified from TCP-C by DEAE-52 cellulose ion exchange resin and dextran gel Sephadex G-200. The structure of polysaccharide TCP-C1-1 was analyzed by high effective gel chromatography， TLC thin layer chromatography， IR and 1H-NMR spectroscopy. The results showed that the TCP-C1-1 polysaccharide isolated and purified from the Tupistra chinensis Baker， was a homogeneous polysaccharide composed of fructose β-(2→1) connection with a relative molecular weight of 1.52 × 104. TCP-C1-1 exhibited good biological activity. Its free radical scavenging rate was able to reach 63.3% at the concentration of 2 mg·mL－1 and its inhibition rate of α-glycosidase was able to reach 78.6% at the concentration of 6.5 mg·mL－1. Besides， the proliferation of mouse monocyte macrophage RAW 264.7 (p〈0.05) was significantly promoted while the NO release induced by LPS was inhibited at the concentration of 1.5 μ g·mL－1. TCP-C1-1 is a natural inulin fructan isolated from Tupistra chinensis Baker for the first time， which provides a certain reference for the preliminary study and the development of polysaccharide components of Tupistra chinensis Baker.
2022, Vol. 56 
