Abstract：Lung cancer has been one of the leading causes of cancer death worldwide， and is prone to recurrence and metastasis (bone metastasis， etc.). In this study， A549-Luc-GFP-Puro cells were screened to establish the bone metastasis model of lung cancer in vivo. After day 7 of injection， obvious tumor signal showed in the tibial cavity by vivo bioluminescent imaging. As the tumor signal increased， the mice lost weight and had persistent pain of leg with reduction of 50% pain threshold. HE staining indicated that the bone marrow cavity and trabecular were filled with highly proliferative tumor cells， and the bone structure was seriously damaged. Mta1 and Mta3， metastasis related genes， up-regulated in bone metastasis tumor. This bone metastasis model has similar biological characteristics and pathological manifestations with bone metastasis of human lung cancer. This model would be used to study the mechanism of bone metastasis of lung cancer and evaluate the efficacy of drugs， which is able to monitor the cancer growth dynamically and non-invasively at multiple time-points， and has highly medical value with high sensitivity and low experimental cost.
2023, Vol. 57 
