Optimization of aerobic growth conditions of a Stutzerimonas stutzeri agent and its partial denitrification performance
CAI Yuheng， CHENG Kai
(Hubei Key Laboratory of Ecological Restoration for River-Lakes and Algal Utilization， College of Resources and Environmental Engineering， Hubei University of Technology， Wuhan 430068， China)
Abstract：Although partial denitrification is one of the hotspots in the researchfield of biological nitrogen removal technology，the study on the microbial agent for partial denitrification is still scarce. In this paper，a strain of Stutzerimonas stutzeri (also known as Pseudomonas stutzeri) WH-01 with both heterotrophic ammonia-oxidation ability and partial denitrification ability was isolated from the activated sludge of a sewage treatment plant. Through the single factor optimization experiments，the optimization of aerobic growth conditions of the WH-01 agent under aerobic condition were determined as： 300 mg·L-1 ammonia nitrogen as nitrogen source,1 500 mg·L-1 glucose as carbon source，culture temperature of 35 ℃ and initial pH of 8.0. When using sodium acetate as the carbon source， although WH-01 agent had the ability of full denitrification， it conducted partial denitrification more preferentially with the nitrite accumulation efficiency of 81% in culture medium and 97% in actual sewage. This study indicated that WH-01 bactericide had the potential of engineering application.
蔡雨衡,程 凯. 施氏假单胞菌菌剂的好氧生长条件优化及其短程反硝化性能[J]. 华中师范大学学报(自然科学版), 2024, 58(2): 184-196.
CAI Yuheng,CHENG Kai. Optimization of aerobic growth conditions of a Stutzerimonas stutzeri agent and its partial denitrification performance. journal1, 2024, 58(2): 184-196.
2024, Vol. 58 
