Abstract：In this study， the endophytic fungus isolated from the roots of Munronia delavayi Franch. was determined as Fusarium oxysporum based on fungus's morphology and 18S rDNA sequence analysis. The isolation and purification of the ethyl acetate extract of fermentation was performed with medium-pressure column chromatography and preparative HPLC. The structures of the nine compounds were identified by extensive spectroscopic analysis as 1HNMR， 13CNMR and LC-MS. Their structures were elucidated as 4-amino-2-hydroxypyrimidinel (1)， 4,5-Dihydro-3H-pyrazole-3-carboxylic acid methyl (2)， 4-hydroxy-7-(2-hydroxy-butyl)-3-methyl-oxepan-2-ketone (3)， 2-amino-3-methyl-pentanoic acid (4)， 2,5-diamino-pentanoic acid (5)， 2-amino-3-methyl-butyric acid (6)， 4-hydroxy-benzoic acid (7)， dehydrofusaric acid (8) and fusaric acid (9). Compound 8 exhibited cytotoxic activity against HepG2 and Hep3B cell lines with IC50 values of 23.9 and 29.1 μg/mL， respectively. However， the cytotoxicity was not shown in L-02 of normal liver cells.
2018, Vol. 52 
