De novo transcriptome assembly and annotation of the leaves of Cyclocarya paliurus
JIANG Xianghui 1,2， YUAN Jing2， WANG Xiang 1,2
1.Qiandongnan Engineering and Technology Research Center for Comprehensive Utilization of National Medicine， Kaili University， Kaili， Guizhou 556011， China；2.College of Chemistry and Materials Engineering， Kaili University， Kaili， Guizhou 556011， China
Abstract：Cyclocarya paliurus are widely used as a traditional Chinese medicine. There are various bioactive secondary metabolites especially in the flowers of Cyclocarya paliurus， such as organic acids， flavones， saponins， iridoids， essential oil， and inorganic elements. The molecular mechanism for the differences is poorly understood so far. In the present paper， the transcriptome of Cyclocarya paliurus leaves was sequenced using Illumina Hiseq 4000 platform， and 50,126 unigenes were generated with an average length of 1,247 bp. 19,875 (39.65%) unigenes were annotated by NCBI Non-Redundant database， and 23,716 (47.31%) unigenes were classified into Gene Ontology (GO) database， along with 14,950 (29.82%) unigenes assigned to Clusters of Orthologous Group (COG) categories. Further analysis results showed 6,012 (11.20%) unigenes were mapped onto 254 pathways in Kyoto Encyclopedia of Genes and Genomes (KEGG)， among which 1,212 unigenes were identified to be involved in biosynthesis of secondary metabolites. It was also found that 126 unigenes are involved in the metabolism of isoprene， including the metabolic pathways of terpenes， citronellal， furanones and glycosides. In addition， a total of 21,089 putative simple sequence repeats (SSRs) were detected. The results of transcriptome analysis of old leaves and young leaves showed that the up-regulation of gene expression in the leaves of Cyclocarya paliurus is involved in the metabolism of steroids， peptides， coenzymes， vitamins and amino acid， while the down-regulation of gene expression is mainly related to signal transmission， lipid metabolism and energy metabolism. This transcriptome dataset might provide some references to understand the biosynthesis and regulation of important bioactive components in Cyclocarya paliurus.
蒋向辉,苑 静,王 翔,. 青钱柳叶片转录组数据组装及基因功能注释[J]. 华中师范大学学报(自然科学版), 2018, 52(6): 822-831.
JIANG Xianghui,YUAN Jing,WANG Xiang,. De novo transcriptome assembly and annotation of the leaves of Cyclocarya paliurus. journal1, 2018, 52(6): 822-831.
2018, Vol. 52 
