Abstract:To establish a method for simultaneously determination for the content of five main flavonoids (luteolin, flavonoid glycoside, kaempferol, quercetin and isoquercitrin) in Cyclocarya paliurus by single marker, which is feasible and accurate. Taking luteolin as internal standard substance, to establish a method for quantitative analysis of multi-components with single marker. To determinate the content of luteolin, flavonoid glycoside, kaempferol, quercetin and isoquercitrin in Cyclocarya paliurus. The contents in 5 batches of samples came from 3 different regions were determined by both external standard method and quantitative analysis multi-components by single marker(QAMS). The scientificity and feasibility of the method were evaluated by comparison of the quantitative results between external standard method and QAMS. The results showed that the relative correction factor (RCF) was perfect. The results calculated with a single marker were consistent with the results by the external standard method. The method with a single marker is accurate and feasible to evaluate the quality of Cyclocarya paliuru.
蒋向辉,蒋天智,苑 静,王 翔. 一测多评法测定青钱柳中5种黄酮类成分含量[J]. 华中师范大学学报(自然科学版), 2017, 51(5): 620-625.
JIANG Xianghui,JIANG Tianzhi,YUAN Jing,WANG Xiang. Determination of five main flavonoids in content in Cyclocarya paliurus by multi-components with single marker. journal1, 2017, 51(5): 620-625.