Tissue culture and rapid propagation system of Rhododendron obtusum ‘Yanzhimi’
ZHOU Yuan1， YANG Pin 1,2， MAO Jing1， TONG Jun1， XU Dongyun1， DONG Yanfang1， FANG Linchuan1， XU Lin1， ZHANG Donglin3
(1. Institute of Forestry & Fruit-tree， Wuhan Academy of Agricultural Science &Technology/Hubei Engineering Technology Research Center of Landscape Plant， Wuhan 430075， China；2. College of Horticulture & Forestry Sciences， Huazhong Agricultural University， Wuhan 430075， China； 3. College of Agricultural & Environmental Sciences， University of Georgia， Athens 30602， USA)
Abstract：Taking the new stems of Rhododendron obtusum ‘Yanzhimi' as explants and WPM as the basic medium， the effects of different growth regulators on bud induction， proliferation and rooting of Rhododendron tissue culture were studied. The results showed that the best medium for inducing axillary buds in the start-up culture was WPM＋0.1 mg·L－1 IBA＋1 mg·L－1 ZT， with the average induction rate of axillary buds of 43.67% and the proliferation coefficient of 1.18. The combination of NAA＋TDZ and NAA＋ZT was used for the proliferation and strong seedling culture， respectively， and the two types exhibited different states in terms of growth and induction. Rhododendron had the best rooting effect on 1/2 WPM basic medium supplemented with 0.5 mg·L－1 NAA and 0.1 mg·L－1 IBA， with a rooting rate of 82.5%. This research laid provide references for production of high-quality seedlings， establishment of genetic transformation system and transgenic breeding of Rhododendron.
2022, Vol. 56 
