Study on extraction technology and liver protective activity of stem and leaf of Sabia parviflora Wall. ex Roxb
ZHANG Xuexue1， ZHAO Dongxiao2， LI Jianqiao1， LU Shunguang2， HU Jianzhong2， LI Manshu1， LIU Zhaoxia1， ZOU Kun1
1.Hubei Key Laboratory of Natural Products Research and Development， College of Biological and Pharmaceutical Sciences， China Three Gorges University， Yichang， Hubei 443002， China；2.Soil and Water Conservation Centre， the Ministry of Water Resources， Beijing 100038， China
Abstract：To optimize extraction conditions of stem and leaf from Sabia parviflora Wall. ex Roxb， in this paper， orthogonal design was used and the ratio of material to liquid， extraction temperature and extraction time were selected as the extraction factors. The survival rate of normal human hepatic cell (LO2) damaged by carbon tetrachloride was considered as the index of hepatoprotective activity for statistical analysis. The results showed that the optimum extraction conditions were as follows： 10 g powder， 200 mL water and 80 ℃ water bath for 1 h. The extraction process is reasonable and repeatable， which would be applied in the extraction process of Sabia parviflora Wall. ex Roxb extraction.
张学学,赵东晓,李建桥,卢顺光,胡建忠,李曼姝,刘朝霞,邹 坤. 小花清风藤茎叶提取工艺优化及保肝活性研究[J]. 华中师范大学学报(自然科学版), 2020, 54(1): 65-69.
ZHANG Xuexue,ZHAO Dongxiao,LI Jianqiao,LU Shunguang,HU Jianzhong,LI Manshu,LIU Zhaoxia,ZOU Kun. Study on extraction technology and liver protective activity of stem and leaf of Sabia parviflora Wall. ex Roxb. journal1, 2020, 54(1): 65-69.
2020, Vol. 54 
