Study on inhibiting hepatocarcinoma cells proliferation of petroleum ether extract from Inula racemosa by accelerated solvent extraction in vitro
XU Chan1， HUANG Huiqi3， LYU Yibing3， LIN Qinxiong3， SONG Ping2， YANG Xinzhou3
1.Department of Pharmacy， TongjiHospital，Tongji Medical College， Huazhong University of Science and Technology，Wuhan 430030， China；2.Division of Science & Technology， Qinghai University for Nationalities， Xining 810007， China；3.School of Pharmaceutical Sciences， South-Central University for Nationalities， Wuhan 430074， China
Abstract：To adequately utilize and develop the Tibetan medicinal resources， we investigated the anticancer activities of extracts derived from Inula racemosa(IR)， and explored the potential mechanism.The accelerated solvent extraction method was used to prepare petroleum ether， ethyl acetate and methanolextracts from I.racemosa. MTT assay， cell morphology， Hoechst 33258 cell staining and flow cytometrywere performed to analysis the anticancer activities of the extracts and the potential mechanisms.The petroleum ether fraction from IR (IR-Pe) showed significant inhibitory activity against HepG2 and Hep3B cell lines with its IC50 values of 21.9 ± 2.3 μg·mL－1， 27.6 ± 3.2 μg·mL－1， and displayed no toxicity against normal liver cells L-02 up to 100 μg·mL－1. IR-Pe exhibited significant time-and dose-dependent cytotoxicity against HepG2 and Hep3B hepatocarcinoma cell lines. Furthermore，IR-Peobviously induced apoptosis of HepG2 and Hep3B cells by cell morphology and flow cytometry. Western blot analysis revealed that the expressions of Bax and caspase-3 increased while Bcl-2 decreased with the increase of dosage of IR-Pe. These indicated that IR-Pe suppressed hepatocellular carcinoma cells through inducing mitochondrial apoptosis and had great potential on drug development and application.
2020, Vol. 54 
