Cytotoxic chemical constituents of Sedum lineare Thunb
YANG Jie1， WEN Yanzhang1， MA Xinhua1， DENG Shihao1， HUANG Yun1， LIN Qinxiong1， WAN Dingrong1， YANG Xinzhou1， SONG Ping2
1.College of Pharmacy， South-Central University for Nationalities， Wuhan 430074， China；2.Division of Science & Technology， Qinghai University for Nationalities， Xining 810007， China
Abstract：Bioassay-guided purification on the methanol extract of the traditional Chinese ethnic medicine “Fojiacao” (Sedum lineare Thunb) was performed on the medium pressure column chromatograph and preparative HPLC. Six compounds has been isolated and the 1H- and 13C-NMR data of kaempferol 7-O-6″-O-malonyl-β-D-glucopyranoside (1) was firstly confirmed. The anti-HCC activities of compounds 1～6 were evaluated by the MTT assay using HepG2 and Hep3B hepatocellular carcinoma cell lines. All compounds exhibited anticancer activities against HepG2 and Hep3B tumor cell lines with IC50 value range of 22.6～91.5 μg/mL， respectively， and showed no cytotoxity against L－02 cell line up to 100 μg/mL. Phenylpropionic acids 3～6 displayed higher anti-HCC activity against HepG2 and Hep3B tumor cell lines with IC50 value range between 22.6 and 44.3 μg/mL than flavonoids 1， 2 in which the cis phenylpropanoid acid 5 exhibited the strongest anti-HCC activity against HepG2 and Hep3B tumor cell lines with IC50 values of 22.6 and 28.2 μg/mL， respectively.
2018, Vol. 52 
