1.School of Chemistry， Chemical Engineering and Life Sciences， Wuhan University of Technology， Wuhan 430070；2.Wuhan Jianmin Zhongwei medicine CO.， Ltd.，Wuhan 430056；3.School of Pharmaceutical Science， South-Central University for Nationalities， Wuhan 430074
Abstract：This paper aims at studying the antitussive effect and antioxidative effect of glycyrrhizin on post-infection cough (pulmonary yin deficiency syndrome) mice.The PIC (pulmonary yin deficiency syndrome) model mice were established by cigarette-smoking exposure， intranasaladministration with small dose of LPS， thyroid hormone and capsaicin atomization method.The mice were randomly divided into control group (physiological saline)， model group (physiological saline)， positive group (montelukast sodium) and glycyrrhizin low， medium， and high dose groups after successful modeling.The behavioral and body mass changes of mice were observed before and after modeling.The sensitivity and number of coughs in mice were determined by capsaicin aerosolized method.Enzyme-linked immunosorbent assay (ELISA) was used to determine levels of inflammation in mouse serum and changes of protein levels associated with antioxidant damage in lung tissue.Hematoxylin-eosin (HE) staining method was used to observe the pathological changes of lung tissue in each group of mice.The results showed that the mechanism of glycyrrhizin in relieving cough might due to its effectively reduction of the release of TNF-α， IL-6， IL-13， TLR2， TGF-β1 and IgE in serum of mice.The airway infection and allergic inflammatory response environment are reduced to attenuate the high sensitivity of cough， and the stimulation of cough center is weakened to relieve the cough.The mechanism of antioxidation might be its ablility on increasing the content of SOD and decreasing the content of MDA in the lung tissue of mice， which improving the disturbance of the antioxidant balance system in vivo，removing excessive free radicals and increasing the content of antioxidant enzymes in the body.
2020, Vol. 54 
