Bioinformatic study of hepatitis C virus envelope protein E1 in Wuhan
ZHOU Xiaodong1， WAN Li1， CAI Guocai2， LEI Xinghua3
1.School of Laboratory Medicine， Chengdu Medical College， Chengdu 610500， China；2.The Third People’s Hospital of Mianyang， Mianyang Sichuan 621000， China；3.Xindu District Hospital of Traditional Chinese Medicine， Chengdu 610500， China
Abstract：The envelope proteins E1 of different HCV genotypes and subtypes in Wuhan were analyzed bioinformatically to predict their secondary structure， nucleotide variability， glycosylation site， hydrophilicity， transmembrane domain， signal peptide， protein modification site， and B cell antigen. The results indicated that envelope proteins E1 of HCVs possess little secondary structure difference； the beginning segment of the sequence is different from the end segment by several amino acid residues， with hypervariable site in the sequence； the genotype 2a shows the highest consistency； the sequence shows significant glycosylation with multiple glycosylation sites； hydrophilicity areas distribute in the sequence and the genotype 1b shows high score； most subtypes of genotypes 1b， 6a， 3b， 3a have one transmembrane domain， while 2a have two； genotypes 1b， 6a， 3b， 3a have no signal peptide， while 2a has； multiple different modification sites and B cell antigens in the protein sequence， which varies with the genotypes and subtypes， exhibiting significant heterogeneity. The study has provided references for demonstrating mechanism of HCV infection and development of region-specific vaccines.
2018, Vol. 52 
