Abstract：Genomic DNA and mRNA were isolated from Aspergillus niger, and the high efficient primers was designed by species similarity. With the methods of PCR and RTPCR respectively, the fragments of the DNA and cDNA of beta-glucosidase gene were obtained. Seqence analysis indicated that DNA fragment was 2 924 bp, and the cDNA sequence was 2 583 bp, which encode 860 amino acids. The identity of the derived protein with Aspergillus niger ffglucosidase has the high homogeneous characteristics of 97%-99%. The foundmental researches for transformation and application of this gene have been achieved in this paper.
收稿日期: 2007-01-25
引用本文:
祝长青,李艳红,周辉,曾献春,赵惠新. 黑曲霉β-葡萄糖苷酶基因的分离[J]. , 2007, 46(1): 0-0.
祝长青,李艳红,周辉,曾献春,赵惠新. Isolation of one β-glucosidase gene in Aspergillus niger. , 2007, 46(1): 0-0.
