Mechanism exploration of xylitol seleniteinduced apoptosis in a human hepatoma cell line SMMC-7221 cells using real-time quantitative PCR
SHEN Hao1， CAO Yu2， LEI Ming1， ZAHID Kashif Rafiq1， WU Xue1，LIU Ke1， LIU Yanli1， LIU Jinlin1， YANG Jihong1， ZHAO Haobin1, QI Chao1
1.Hubei Key Laboratory of Genetic Regulation and Integrative Biology， College of Life Science， Central China Normal University， Wuhan 430079; 2.College of Chemistry， Central China Normal University， Wuhan 430079
Abstract：Xylitol selenite， a novel synthetic compound， which is able to cause apoptosis in human hepatoma cell line SMMC-7221 cells and induce apoptosis following GSH depletion. To provide more investigation on its anticancer mechanisms， the changes of caspase-3， caspase-8 and caspase-9 in mRNA levels in SMMC-7221 cells treated with xylitol selenite were determined using realtime quantitative PCR. After treated with 05 or 1 mg/L xylitol selenite for 12， 24， 36， and 48 h， significant upregulation in the mRNA expression of caspase-3 was observed， comparing with control group. Moreover， expressions of both caspase-8 and caspase-9 mRNA only markedly varied in 24 h， and there were no significant differences at other time points. In contrast to control group， expressions of caspase-3， caspase-8 and caspase-9 mRNA were upregulated at 12 h， reaching its peak at 24 h and beginning to decrease at 36 and 48 h after xylitol selenite treatment.Xylitol selenite， a promising anticancer drug to induce apoptosis in SMMC-7221 cells， may regulate the apoptosis through extrinsic and intrinsic pathway and more studies on its anticancer mechanisms should be determined.
