Abstract:In order to select high efficient Rhizobia strains for Stylosanthes guianensis cv. Reyan No.2 (shorted as Reyan 2) in the acid red soil, 19 Rhizobia strains were inoculated into the roots of Reyan 2. Impact of different Rhizobia strains on Reyan 2 in acid red soil were determined by indexes such as height and dry weight, nitrogen content and phosphorus content of stem and leaf. Then highly efficient strains were identified through principal component analysis. The results showed that highly efficient strains for Stylosanthes guianensis cv. Reyan No.2 in acid red soil are RJS9-1, BS2-2, ZJ2-2, RJS9-2 and FS3-1-1, while the strains not beneficial to plant growth are LY1, NN1-1-2 and SM5-1. These results provided reference in improving Stylosanthes guianensis production by inoculating highly efficient Rhizobia in practice.