Antioxidant and anti-lung cancer activity of ethanol extract from Ophiocordyceps gracilis in Xinjiang
ZAMAN Manzilamu1， HUANG Luodong2， YE Xing1， ABUDUAINI Aifeire1， ZHOU Huiying1， MA Yufeng1， SUO Feiya1
1.College of Life Science and Technology， Xinjiang University， Urumqi 830046， China；2.Department of Ecology， College of Life Science and Technology， Jinan University， uangzhou 510632， China
Abstract：To evaluate the antioxidant capacity of the ethanol extract of fermented mycelium from Ophiocordyceps gracilis in Xinjiang (OGPDC)， the DPPH free radical scavenging ability and total reducing ability were determined. Also， the inhibitory effect of OGPDC on the proliferation of lung cancer cells A549 was analyzed under MTT. The antioxidant results specified that OGPDC embraced better DPPH free radical scavenging ability and total reducing ability， and the DPPH scavenging ability showed relatively high effect with IC50 value of 1.952 mg/mL. Meanwhile， the DPPH scavenging ability and total reducing ability increased with the escalation of OGPDC concentration. MTT results displayed that， to some degree， OGPDC inhibited the proliferation of lung cancer cells A549， and increased with the escalation of OGPDC concentration； the IC50 value of the drug increased to 10.87 μg/mL in 48 h. Additionally， the cell cycle and apoptosis rate of lung cancer cells A549 were detected by flow cytometry in 48 h， and the total apoptosis rate was higher than that of the control group (only added nutrient solution). The highest total apoptotic rate was 34.77%， which made the cycle block of cells A549 blocked in the G0/G1 phase. The results showed that the OGPDC had anti-oxidation and anti-cancer activity in a certain concentration range， suggesting that the fermented mycelium of O. gracilis in Xinjiang owns important development and utilization value.
2019, Vol. 53 
