ICP-MS and GC-MS studies on different parts of Cinnamomum camphora
CHEN Zhenyu1， LI Xiaocong2， ZHANGg Xin3， LIU Chengxiong1， ZOU Kun1， LIU Zhaoxia1
1.Hubei Key Laboratory of Natural Products Research and Development， College of Biological and Pharmaceutica Sciences， China Three Gorges University， Yichang， Hubei 443002， China；2.Yichang Humanwell Pharmaceutical Co.， Ltd.， Yichang Hubei 443002， China；Three Gorges Public Inspection and Testing Center， Yichang Hubei 443002， China
Abstract：Microwave digestion combined with flame atomic absorption spectrometry (FAAS) and microwave digestion inductively coupled plasma mass spectrometry (ICP-MS) are commonly used in the extraction of plant trace elements. The traditional extraction methods of plant volatile oil include steam distillation， solvent extraction， pressing and adsorption， while there are modern extraction methods such as simultaneous distillation and extraction， supercritical CO2 extraction， subcritical water extraction， enzymatic assisted extraction， microwave assisted extraction， ultrasonic assisted extraction， solid phase microextraction， combined extraction. In order to study and compare the components of trace elements and volatile oils in different parts of bark， stem， trunk and leaf of Camphor camphor in Hubei Province the content of trace elements in different parts of camphor tree was determined by inductively coupled plasma mass spectrometry (ICP-MS)， and the volatile oils from different parts of camphor tree were extracted by reflux extraction with petroleum ether as solvent. The relative content of volatile oils was determined by peak area normalization method. The chemical constituents were identified by gas chromatography-mass spectrometry (GC-MS). The results showed that the contents of 14 trace elements in different parts of camphor tree were different， but the contents of Al， Zn and Fe were higher， while the contents of Co， V， Se and Cd were lower. 29， 21， 28 and 9 different compounds were respectively detected from bark， stem and leaf of camphor tree， which were all oxygenates and olefins. The main constituents in bark was safrole (36.23%) and characteristic constituents was 4-isopro pyl-1,6-dimethyl-1,2,3,4,4a,7-hexahydronaphthalene (1.71%). The main constituents in trunk was safrole (22.45%) and characteristic constituents was 1-isopropyl-4,7-dimethyl-1,2,4a,5,8a-hexahydronaphthalene (1.55%). The main constituents in stem was safrole (69.98%) and characteristic constituents was (1R,8a-1)-isopropyl-4,7-dimethyl-1,2,3,5,6,8a-hex ahydronaphthalene (2.25%). The main constituents of leaves were alpha-terpineol (40.73%) and eucalyptol (31.96%) with the characteristic constituents of (1R,4S)-7,7-dimethyl-2-methylenebicyclo[2.2.1]heptane (4.71%). There are differences in trace elements in different parts of Cinnamomum camphora in Yichang， Hubei Province. The content of heavy metal elements in bark， trunk and stem of camphor tree and the content of heavy metal elements in leaves except Cu were in accordance with the limit standard of green industry standard for import and export of medicinal plants and preparations. The volatile oils in different parts of camphor trees differ greatly. The experimental results provide certain experimental basis for the development and utilization of camphor trees in the future.
2019, Vol. 53 
