Antitumor constituents from roots of Ligularia veitchiana
XU Dong1， LIU Dan1， ZHANG Chenhuan1，ZHANG Runfang1， WANG Qinlian1， ZOU Kun1,2， WANG Hui1,2
1.Hubei Key Laboratory of Natural Products Research and Development， College of Biological and Pharmaceutical Sciences， China Three Gorges University， Yichang， Hubei 443002， China； 2.Key Laboratory of Functional Yeast， China National Light Industry， College of Biological and Pharmaceutical Sciences， China Three Gorges University， Yichang， Hubei 443002， China
Abstract：Various chromatographic techniques， including silica gel column chromatography， TLC and HPLC， were used to investigate the chemical constituents of roots of L. veitchiana.Three compounds were isolated and then identified as bakkenolide A (1)， phthalic acid isodibutyl ester (2)， dibutylphthalate (3)， eremophila-7(11),9-dien-8-one (4)， 6β，8β-hydroxyeremophil-7(11)-en-12,8α-olide (5)， 6β-hydroxyeremophil-7(11)-en-12,8β-olide (6)， and 6β-hydroxyeremophil-7(11)-en-12,8α-olide (7) by means of spectroscopic and chemical data. Among them， compounds 1，2，4 and 7 were isolated from this plant for the first time.Compound 1 was the first sesquiterpenoid with a spiro-ring skeleton isolated from L. veitchiana. Compounds 6 and 7 area pair of diastereoisomer. Cytotoxicities of all the compounds against cancer cell lines of HGC-27 were evaluated in vitro by MTT assay， and compound 1 showed a significant growth inhibition of tumor cells HGC-27 with IC50 of 31.10 μg·mL－1.
2021, Vol. 55 
