Abstract：The DNA was extracted by saturated phenol method from VHA273 Strain of Heliothis armigera polyhedrosts virus. Three cell lines from Heliothis armigera (Ha). Spodoptera frugiperda(Sf)and Bombyx mori(Bm)were transfected respectively by the DNAs with calthium phosphate co-precipitation method, The virus particles, nucleocapsids, inclusion bodies and polyhedrin of VHA273 strain could not be detected at all by ELISA, light and electronic microscope. However, the typical densonucleosis symptom and desonucleosis viruses (DNVs)were observed with electronic microscope only in the transfected Hs cell line. This paper considered that the DNVs may originally be potential virus. It is likely that that these potential DNVs were activated when Ha cells were transfected by the VHA273-DNA, the reproduction of VHA273-DNA entering Ha Cells could be repressed in the meantime.
收稿日期: 1991-04-25
引用本文:
吴柏春,王行国,余泽华. 中国棉铃虫核多角体病毒(Heliothis armiqera NPV)VHA273毒株DNA转染传代细胞的研究[J]. , 1991, 30(4): 0-0.
吴柏春,王行国,余泽华. THE STUDIES OF TRASFECTING THREE CELL LINES WITH THE VHA237-DNA OF HELIOTH IS ARMIGERA NPV. , 1991, 30(4): 0-0.
