Abstract：Microcystins are isolated and purified by HPLC from the blue green algal (cyanobacterial) Microcysts bollom collected in Dianchi Lake of the Southwestern China. The Microcystin LR(MCYST LR), YR(MCYST YR) and RR(MCYST RR) which are diluted into three similar concentrations in advance are added to the cultured Hela and Vero tumor cells. The results after incubating for 24 hrs show: 1.all three microcystins represent toxic and inhibitory effect on the growth of the two tumor cells, furthermore, the intensity of the effect is in correspondence with the concentration of the toxins. It is almost the same that the two tumor cells are caused death after 24 hrs with higher concentrations of the three microcystins. The dead tumor cells are irregular and dry. 2.the Hela cells seem to be more poisobned than the Vero cells in the same concentrations of the toxin liquids. 3.Microcystin YR represents more inhibitory effect on the Hela cells than Microcystin RR does. 4.under higher toxin concentration, the observation over long term on the test is ineffective for the shortage of the nutrition and that may be due to the relatively low content of the original microcystins.
收稿日期: 1999-01-25
引用本文:
赵以军 甘小妮. 微囊藻毒素对Hela细胞和Vero细胞的毒性效应[J]. , 1999, 38(1): 0-0.
赵以军 甘小妮. Inhibitory effects of microcystins on Hela and Vero cells in vitro. , 1999, 38(1): 0-0.
