Abstract：A fragment of SeMNPV (Spodoptera exigua multiplenucleocapsid nuclear polyhedrosis virus) about 1.3 kb had been amplified by employing PCR technique and by designing a pair of primers based on the sequence of capsid protein gene vp39 of AcMNPV( Autographa californica multiplenucleocapsid nuclear polyhedrosis virus). The recombinant expression plasmid pET Se39 has been constructed by subcloning the 1.3 kb fragment into the procaryotic expression vector pET 28. After transformed into E.coli BL21, SeMNPV vp39 gene was highly expressed by the inducement of IPTG. SDS PAGE analysis showed that the molecular weight of the expressed protein was about 39 kD.The amount of the expressed product reached to the highest level when it had been induced with IPTG for 4 hours.
收稿日期: 2001-02-25
引用本文:
段媛媛,杨成丽,刘德立,范文韬. 甜菜夜蛾多核壳型核型多角体病毒vp39基因的克隆与表达[J]. , 2001, 40(2): 0-0.
段媛媛,杨成丽,刘德立,范文韬. Cloning and expression of the vp39 gene of Spodoptera exigua multiplenucleocapsid nuclear polyhedrosis virus. , 2001, 40(2): 0-0.
