Abstract： The vip3A gene in a size of 2.3 kb from a wild Bacillus thuringiensis WY-197 was cloned and sequenced. Comparison of the amino acid sequence with those of reported VIPs revealed high similarity. Vip3A-197 gene was subcloned into prokaryotic expression vector pET33 b to form recombinant expression plasmid pEVip and was transfered into escherichia coli, BL21. Recombinant strain of E. coli BL21 induced by IPTG could produce 88 000 protein by SDS-PAGE. Bioassay also showed that vip3A-197 protein was toxic to Spodoptera exigua and Helicoverpa armigera larvae.
收稿日期: 2005-02-25
引用本文:
李江 闫建平 蔡全信 袁志明. 苏云金芽孢杆菌营养期杀虫蛋白基因的克隆、表达及杀虫活性分析[J]. , 2005, 44(2): 0-0.
李江 闫建平 蔡全信 袁志明. Cloning and expression product of vip3A gene from Bacillus thuringiensis and analysis of inseceicidal activity. , 2005, 44(2): 0-0.
