Abstract：Saccharomyces cerevisiae was a desired expression system for eukaryotic protein. Cytochrome P450nor gene was subcloned into the shuttle vector pAUR123 to yield the recombinant plasmid pAUR-P450nor2. The recombinant plasmid pAUR-P450nor2 was transformed into S. cerevisiae AH22 and got positive clone after the screening with Aureobasidin A and colony PCR. The SDS-PAGE showed that the expression product was recombinant cytochrome P450nor2 and got a high level.
收稿日期: 2006-02-25
引用本文:
陈舒丽,刘德立,郑永良,赵莉,张山,邹煜平. 重组真菌细胞色素P450nor2在酵母细胞中的表达[J]. , 2006, 45(2): 0-0.
陈舒丽,刘德立,郑永良,赵莉,张山,邹煜平. The expression of recombinant cytochrome P450nor2 in Saccharomyces cerevisiae. , 2006, 45(2): 0-0.
