Abstract：On the basis of sequence analysis,deletion mutants of phycocyanin lyase CpcE/CpcF in Mastigocladus laminosus were constructed: cpcE(42-276),cpcE(1-274),cpcE(1-272),cpcF(1-160),cpcF(10-213).Gene of the deletion mutants was then cloned into expression vector pET 30a(+).The activities of the expressed proteins were studied through the in vitro reconstitution test,which showed the function of deleted domain in the enzyme catalysis.
收稿日期: 2006-02-25
引用本文:
张玲,武栋,叶斌,周明,赵开弘. 藻蓝蛋白裂合酶突变体的构建及其对催化功能的影响[J]. , 2006, 45(2): 0-0.
张玲,武栋,叶斌,周明,赵开弘. Construction of deletion mutants and its effects on the catalytic function of phycocyanin lyase in Mastigocladus laminosus. , 2006, 45(2): 0-0.
