Protective effect of ginseng polysaccharides on oxidative stress injury in cardiomyocytes by reducing ROS level and apoptosis
TIAN Yaobo1， ZHAO Daqing1， LI Xiangyan1， SUN Liwei2， CHEN Xuenan3
1.Changchun University of Chinese Medicine， Changchun 130017， China；2.Jilin Beihua University， Jilin， Jilin 132013， China；3.Affiliated Hospital of Changchun University of Traditional Chinese Medicine， Changchun 130021， China
Abstract：The aim of this study was to investigate protective effect of ginseng polysaccharides (GPS) on oxidative damage induced by hydrogen peroxide (H2O2) in rat cardiomyocytes H9c2. MTT assay was used to evaluate cell viability. The biochemical parameters of oxidative damage， lactate dehydrogenase (LDH)， superoxide dismutase (SOD) and malonaldehyde (MDA) were measured by testing kits. The percentage of apoptosis and reactive oxygen species (ROS) production in the cells were determined by flow cytometry. And apoptosis-related protein was detected by Western blot. The results showed that GPS (6.25 μg/mL) significantly enhanced cell viability from (57.47±5.08) % to (85.65±4.28) % (P<0.001)， which indicated that GPS protected H9c2 cells from H2O2-induced cytotoxicity. The results from DCFH-DA staining showed that ROS production was decreased by GPS through decreasing the content of MDA and increasing the activity of SOD. Moreover， GPS inhibited H2O2-induced apoptosis through increasing the ratio of Bcl-2/Bax and reducing the level of apoptosis-related protein. We concluded that GPS protected cardiacmyocytes from oxidative stress injury though reducing ROS production and apoptosis， which provided theories foundation for molecular mechanism of GPS and the development of ginseng-related products for the protection of heart injury.
2018, Vol. 52 
