Study on compositions and antibacterial activities of the total flavonoids from the tender leaves of Ampelopsis grossedentata (Hand.-Mazz.) W. T. Wang in Lichuan
LI Yu1， LIU Cuijun1， LIAO Lujin1， GUO Hanjiu1， RAN Yalan2， LIU Dan3， LUO Jianqun4， HE Meijun1
1.Institute of Chinese Herbal Medicines， Hubei Academy of Agricultural Sciences， Enshi， Hubei 445000， China；2.Agricultural and Rural Bureau of Enshi Tujia and Miao Autonomous Prefecture， Enshi， Hubei 445000， China；3. Medical School， Hubei Minzu University， Enshi， Hubei 445000， China； 4. Enshi Selenium Commander and Ecological Agriculture Company， Enshi， Hubei 445000， China
Abstract：The total flavonoids from the tender leaves of Ampelopsis grossedentata (vine tea) were extracted by ultrasonic. The total flavonoids were identified by LC-HR-ESIMS， and the content of each component was determined by external standard method. The antibacterial activity of total flavonoids against 10 clinical drug-resistant bacterias in vitro by filter paper method. The results showed that 7 flavonoids were identified from the total flavonoids in the tender leaves. The main components of total flavonoids were dihydromyricetin (18.57%)， myricitrin (0.53%)， myricetin (1.29%)， taxifolin (1.38%)， trifolin (0.78%)， kaempferol (0.45%)， and quercetin (0.51%). The total flavonoids of vine tea had medium inhibitory activity against bacillus thuringiensis， methicillin-resistant staphylococcus aureus， bacillus subtilis and vibrio alginolyticus XSBZ14， with the minimum inhibitory concentration (MIC) value of 1.0 mg·mL－1，2.0 mg·mL－1,2.0 mg·mL－1 and 1.0 mg·mL－1， respectively.
李 宇,刘翠君,廖璐婧,郭汉玖,冉亚兰,刘 丹,罗建群,何美军. 利川产区显齿蛇葡萄嫩叶总黄酮成分和抗菌活性研究[J]. 华中师范大学学报(自然科学版), 2020, 54(5): 820-825.
LI Yu,LIU Cuijun,LIAO Lujin,GUO Hanjiu,RAN Yalan,LIU Dan,LUO Jianqun,HE Meijun. Study on compositions and antibacterial activities of the total flavonoids from the tender leaves of Ampelopsis grossedentata (Hand.-Mazz.) W. T. Wang in Lichuan. journal1, 2020, 54(5): 820-825.
2020, Vol. 54 
