Abstract：In order to investigate the effects of fruA on the growth， virulence and biofilm formation of Salmonella enterica serovar Typhi (S. Typhi)， the overexpressing strain of fruA (WT-pfruA) and its empty vector strain (WT-pBAD33) were prepared by pBAD33. The effects of fruA on the growth， virulence and biofilm formation of S. Typhi were detected by bacterial growth curve assay， swimming assay， T84 cell invasion assay and 96-well microplate crystal violet staining. At the same time， qRT-PCR was used to analyze the regulation of fruA on the expression of virulence and biofilm related genes. The results showed that The WT-pfruA and WT-pBAD33 strains of S. Typhi were prepared successfully. After overexpression of fruA， the growth of S. Typhi did not change significantly， while the motility， invasion and biofilm formation ability increased. Similarly， compared with the control strain， the mRNA level of motility， invasion and biofilm related genes in WT-pfruA were significantly up-regulated. In conclusion， the fruA gene of S. Typhi was able to enhance the virulence and biofilm formation， and positively regulate its phenotype related genes.
2023, Vol. 57 
