Abstract:531 students of four universities from the first to the Four grade were investigated, by using self-made questionnaire on college students' psychological conflict, Cawte's stress questionnaire and subjective well-being questionnaire. Results: (1) The higher frequencies of conflict resulted in the higher stress levels both physic and mental. (2) The frequencies of psychological conflict was positively related to stress level ( r=0.533,P<0.01). (3) It negatively related to subjective well-being ( r=-0.158,P<0.01). Conclusion: As one of the stressors, the higher frequencies of conflict was one of the important factors on the mental health in the college students. According to these results, it is necessary to concern with the current status of students' conflict.