甜菜夜蛾核型多角体病毒vp39基因重组真核表达质粒的构建及鉴定

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摘要利用DNA重组技术，将杆状病毒极早期基因iel启动子基因克隆至重组质粒pGEM-Se39中，成功地构建了重组真核表达质粒pGEM-IE1-Se39。

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Abstract：The baculovirus immediately early gene ie1 promoter was subcloned into recombinant plasmid pGEM-Se39 by using DNA recombinant technique. After transformation, the recombinant plasmid was isolated and digested with different endo-restriction enzymes. The result showed that the recombinant eukaryotic expression plasmid pGEM-IE1-Se39 containing an ie1 promotor was constructed successfully.

收稿日期: 2002-03-25

引用本文:

段媛媛,杨成丽,周建刚,范文韬,彭建新,江月,刘德立. 甜菜夜蛾核型多角体病毒vp39基因重组真核表达质粒的构建及鉴定[J]. , 2002, 41(3): 0-0.
段媛媛,杨成丽,周建刚,范文韬,彭建新,江月,刘德立. Construction and identification of the recombinant eukaryotic expression plasmid pGEM-IE1-Se39. , 2002, 41(3): 0-0.

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http://journal.ccnu.edu.cn/zk/CN/ 或 http://journal.ccnu.edu.cn/zk/CN/Y2002/V41/I3/0