Abstract：The antioxidant activity of aqueous extract of Clerodendrum bungei was evaluated by DPPH free radical scavenging experiment. DPPH-HPLC-QTOF-MS/MS was developed for rapid screening and identification of antioxidants. After reaction with DPPH radical， HPLC peak of thirteen antioxidants in Clerodendrum bungei extract disappeared. By analysis of their retention times， characteristic ultraviolet spectra， fragmentation pathways， diagnostic product ions， and in comparison with standards， structures of thirteen antioxidants were characterized or tentatively identified， including twelve phenylglycosides and caffeic acid. Among them， eight phenylglycosides were firstly reported in Clerodendrum genus， and caffeic acid was found in Clerodendrum bungei at the first time. The developed method had the potential to be a high throughput tool for rapid， sensitive， and accurate screening of antioxidants compounds from complex matrix without tedious purification procedures， which could then be used for quality control.
邓清平,侯光菡. DPPH-HPLC-QTOF-MS/MS快速筛选和鉴定臭牡丹中抗氧化活性成分[J]. 华中师范大学学报(自然科学版), 2018, 52(6): 816-821.
DENG Qingping,HOU Guanghan. Rapid screening and identification of antioxidants from Clerodendrum bungei by DPPH-HPLC-QTOF-MS/MS. journal1, 2018, 52(6): 816-821.
2018, Vol. 52 
